The Electrometric Titration of Hemin and Hematin
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چکیده
It was shown 5 years ago (1) that the change from methemoglobin to hemoglobin could be studied by an electrochemical method. The reduction by means of sodium hydrosulfite and the oxidation of the reduced compound (hemoglobin) by ferricyanide were found to involve 1 equivalent. These results have been confirmed and extended in later work (2). An application of the same method to a study of the reduction of alkaline hematin solutions (1) yielded uncertain results which seemed to indicate that 2 equivalents of hydrogen were involved in the reduction of this compound. No definite conclusion could be drawn from the data, however. A continuation of this work has now shown that satisfactory electrochemical titration curves may be obtained if titanous tartrate is employed as the reducing agent. The results are very definite and show that the change involves only 1 equivalent. While this work was in progress, two other workers reported results obtained by entirely different methods. These show that the reduction of hematin, like the reduction of methemoglobin, is the change from a ferric to a ferrous compound. The experiments which we now have to report, therefore, serve merely to confirm the recent observations of Haurowitz (3) and those of Hill (4).
منابع مشابه
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تاریخ انتشار 2003